Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503865
Title Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK
Author(s) Day, Michaela J.; Rodríguez, Irene; Essen-Zandbergen, Alieda van; Dierikx, Cindy; Kadlec, Kristina; Schink, Anne Kathrin; Wu, Guanghui; Chattaway, Marie A.; DoNascimento, Vivienne; Wain, John; Helmuth, Reiner; Guerra, Beatriz; Schwarz, Stefan; Threlfall, John; Woodward, Martin J.; Coldham, Nick; Mevius, Dik; Woodford, Neil
Source Journal of Antimicrobial Chemotherapy 71 (2016)5. - ISSN 0305-7453 - p. 1178 - 1182.
DOI http://dx.doi.org/10.1093/jac/dkv485
Department(s) CVI Bacteriology and Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract

Objectives: This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. Methods: A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. Moreover, IncI1-Iγ and IncN plasmids were characterized by plasmid MLST. Results: The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. Overall, blaCTX-M-1 was the most frequently detected ESBL gene, followed by blaCTX-M-15, which was the most common ESBL gene in the human isolates. The most common plasmid replicon type overall was IncI1-Ig followed by multiple IncF replicons. Conclusions: ESBL genes were present in a wide variety of E. coli STs. IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts.

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