Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 504236
Title Interaction between the moss Physcomitrella patens and Phytophthora : A novel pathosystem for live-cell imaging of subcellular defence
Author(s) Overdijk, Elysa J.R.; Keijzer, Jeroen De; Groot, Deborah De; Schoina, Charikleia; Bouwmeester, Klaas; Ketelaar, Tijs; Govers, Francine
Source Journal of Microscopy 263 (2016)2. - ISSN 0022-2720 - p. 171 - 180.
DOI http://dx.doi.org/10.1111/jmi.12395
Department(s) Laboratory of Phytopathology
Laboratory of Cell Biology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Live-cell imaging - Physcomitrella patens - Phytophthora capsici - Phytophthora infestans - Plant-pathogen interaction - Subcellular defence
Abstract

Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.

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