Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 504580
Title Ternary supramolecular quantum-dot network flocculation for selective lectin detection
Author(s) Oikonomou, Maria; Wang, Junyou; Carvalho, Rui Rijo; Velders, Aldrik H.
Source Nano Research 9 (2016)7. - ISSN 1998-0124 - p. 1904 - 1912.
DOI https://doi.org/10.1007/s12274-016-1082-1
Department(s) BioNanoTechnology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) cyclodextrin - flocculation - lectin - quantum dot - sensor - supramolecular
Abstract

We present a versatile, tuneable, and selective nanoparticle-based lectin biosensor, based on flocculation of ternary supramolecular nanoparticle networks (NPN), formed through the sequential binding of three building blocks. The three building blocks are β-cyclodextrin-capped CdTe quantum dots, tetraethylene glycol-tethered mannose-adamantane cross-linkers (ADTEGMan), and the tetravalent lectin Concanavalin A (ConA). The working principle of this selective sensor lies in the dual orthogonal molecular interactions of the linker, uniting adamantane-β-cyclodextrin and mannose-lectin interaction motifs, respectively. Only when the lectin is present, sequential binding takes place, leading to in situ self-organization of the sensor through the formation of ternary supramolecular networks. Monitoring the loss of fluorescence signal of the quantum dots in solution, caused by controlled network formation and consecutive flocculation and sedimentation, leads to selective, qualitative, and quantitative lectin detection. Fluorescent sedimented networks can be observed by the naked eye or under UV illumination for a lectin concentration of up to 10−8 M. Quantitative detection is possible at 100 min with a lower detection limit of approximately 5 × 10−8 M.[Figure not available: see fulltext.]

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