|Title||Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution|
|Author(s)||Boggs, Irina; Hine, Brad; Smolenksi, Grant; Hettinga, Kasper; Zhang, Lina; Wheeler, Thomas T.|
|Source||Data in Brief 8 (2016). - p. 52 - 55.|
Food Quality and Design
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Dairy - Drying off - Host defence - Proteomics|
Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication "Changes in the repertoire of bovine milk proteins during mammary involution" (Boggs et al., 2015) . Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.