Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 504615
Title Towards a peptide-based suspension array for the detection of pestivirus antibodies in swine
Author(s) Wal, Fimme J. van der; Jelsma, Tinka; Fijten, Helmi; Achterberg, René P.; Loeffen, Willie L.A.
Source Journal of Virological Methods 235 (2016). - ISSN 0166-0934 - p. 15 - 20.
DOI http://dx.doi.org/10.1016/j.jviromet.2016.04.022
Department(s) CVI Infection Biology
CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Classical swine fever virus - Peptide - Serum - Suspension array
Abstract

Classical swine fever (CSF) is a highly contagious and lethal disease in swine. Serological tests for the diagnosis of CSF need not only to detect antibodies against CSFV, but also need to differentiate these from antibodies against other pestiviruses. To investigate the possibilities of specific peptide-based serology, various synthetic peptides that represent a well-described linear epitope of the CSFV E2 protein (TAVSPTTLR) were used to test the viability of a peptide-based suspension array for the detection of antibodies against pestiviruses in swine.The results show that N-terminally biotinylated peptides can bind to avidin conjugated beads, and function in detection of the corresponding monoclonal antibody WH303. There are indications that the length of the spacer between epitope and biotin affect the efficiency of the peptide-antibody interaction. A protocol was established that enables probing for antibodies in porcine sera, where neutravidin-blocking of serum and the use of empty control beads for normalization was crucial. With a set of porcine sera with antibodies against various pestiviruses, the proof of concept of a peptide-based suspension array for specific detection of antibodies against pestiviruses in porcine sera was demonstrated.

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