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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 505042
Title Combined epigallocatechin-3-gallate and resveratrol supplementation for 12 wk increases mitochondrial capacity and fat oxidation, but not insulin sensitivity, in obese humans: a randomized controlled trial
Author(s) Most, Jasper; Timmers, S.; Warnke, I.; Jocken, J.J.W.; Boekschoten, M.V.; Groot, Philip de; Bendik, Igor; Schrauwen, Patrick; Goossens, Gijs H.; Blaak, Ellen E.
Source American Journal of Clinical Nutrition 104 (2016)1. - ISSN 0002-9165 - p. 215 - 227.
Department(s) Chair Nutrition Metabolism and Genomics
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract Background: The obese insulin-resistant state is characterized by
impairments in lipid metabolism.We previously showed that 3-d supplementation
of combined epigallocatechin-3-gallate and resveratrol
(EGCG+RES) increased energy expenditure and improved the
capacity to switch from fat toward carbohydrate oxidation with
a high-fat mixed meal (HFMM) test in men.
Objective: The present study aimed to investigate the longer-term
effect of EGCG+RES supplementation on metabolic profile, mitochondrial
capacity, fat oxidation, lipolysis, and tissue-specific insulin
Design: In this randomized double-blind study, 38 overweight
and obese subjects [18 men; aged 38 6 2 y; body mass index
(kg/m2): 29.7 6 0.5] received either EGCG+RES (282 and
80 mg/d, respectively) or placebo for 12 wk. Before and after the
intervention, oxidative capacity and gene expression were assessed
in skeletal muscle. Fasting and postprandial (HFMM) lipid metabolism
was assessed by using indirect calorimetry, blood sampling,
and microdialysis. Tissue-specific insulin sensitivity was assessed
by a hyperinsulinemic-euglycemic clamp with [6,6-2H2]-glucose
Results: EGCG+RES supplementation did not affect the fasting
plasma metabolic profile. Although whole-body fat mass was not
affected, visceral adipose tissue mass tended to decrease after the
intervention compared with placebo (P-time 3 treatment = 0.09).
EGCG+RES supplementation significantly increased oxidative capacity
in permeabilized muscle fibers (P-time 3 treatment ,
0.05, P-EGCG+RES , 0.05). Moreover, EGCG+RES reduced fasting
(P-time 3 treatment = 0.03) and postprandial respiratory quotient
(P-time 3 treatment = 0.01) compared with placebo. Fasting and
postprandial fat oxidation was not significantly affected by EGCG
+RES (P-EGCG+RES = 0.46 and 0.38, respectively) but declined
after placebo (P-placebo = 0.05 and 0.03, respectively). Energy expenditure
was not altered (P-time 3 treatment = 0.96). Furthermore,
EGCG+RES supplementation attenuated the increase in plasma
triacylglycerol concentrations during the HFMM test that was observed
after placebo (P-time 3 treatment = 0.04, P-placebo =
0.01). Finally, EGCG+RES had no effect on insulin-stimulated glucose
disposal, suppression of endogenous glucose production, or
Conclusion: Twelve weeks of EGCG+RES supplementation increased
mitochondrial capacity and stimulated fat oxidation compared
with placebo, but this did not translate into increased tissue-specific
insulin sensitivity in overweight and obese subjects. This trial was
registered at as NCT02381145. Am J Clin Nutr
doi: 10.3945/ajcn.115.122937.
Keywords: insulin sensitivity, mitochondrial capacity, obesity,
polyphenols, resveratrol
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