|Title||Selection of oleaginous yeasts for fatty acid production|
|Author(s)||Lamers, Dennis; Biezen, Nick van; Martens, Dirk; Peters, Linda; Zilver, Eric van de; Jacobs-van Dreumel, Nicole; Wijffels, René H.; Lokman, Christien|
|Source||BMC Biotechnology 16 (2016)1. - ISSN 1472-6750|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Lipid production - Oleaginous yeast - Schwanniomyces occidentalis - TAG|
Background: Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels, which are based on non-food competing "waste carbon sources". Results: In this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were: a broad maximum temperature and pH range for growth (robustness of the strain), a broad spectrum of carbon sources that can be metabolized (preferably including C-5 sugars), a high total fatty acid content in combination with a low glycogen content and genetic accessibility. Conclusions: Based on these selection criteria, among 24 screened species, Schwanniomyces occidentalis (Debaromyces occidentalis) CBS2864 was selected as a promising strain for the production of high amounts of lipids.