|Title||Effect of osmopriming on germination and initial growth of physalis angulata L. under salt stress and on expression of associated genes|
|Author(s)||Souza, Manuela Oliveira de; Pelacani, Claudinéia R.; Willems, Leo A.J.; Castro, Renato D. de; Hilhorst, Henk W.M.; Ligterink, Wilco|
|Source||Anais da Academia Brasileira de Ciencias 88 (2016)S1. - ISSN 0001-3765 - p. 503 - 516.|
Laboratory of Plant Physiology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Germinability - RT-qPCR - Salinity - Seedling growth|
This study aimed to evaluate the effects of priming on seed germination under salt stress and gene expression in seeds and seedlings of P. angulata L. After priming for 10 days, seed germination was tested in plastic trays containing 15 ml of water (0 dS m-1 - control) or 15 ml of NaCl solution (2, 4, 6, 8, 10, 12, 14 and 16 dS m-1). Fresh and dry weight of shoots and roots of seedlings were evaluated at 0, 2, 4, 6, 8 dS m-1. Total RNA was extracted from whole seeds and seedlings followed by RT-qPCR. The target genes selected for this study were: ascorbate peroxidase (APX), glutathione-S-transferase (GST), thioredoxin (TXN), high affinity potassium transporter protein 1 (HAK1) and salt overly sensitive 1 (SOS1). At an electroconductivity of 14 dS m-1 the primed seeds still germinated to 72%, in contrast with the non-primed seeds which did not germinate. The relative expression of APX was higher in primed seeds and this may have contributed to the maintenance of high germination in primed seeds at high salt concentrations. GST and TXN displayed increased transcript levels in shoots and roots of seedlings from primed seeds. Priming improved seed germination as well as salt tolerance and this is correlated with increased expression of APX in seeds and SOS1, GST and TXN in seedlings.