Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 506373
Title Inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of Rice stripe virus
Author(s) Liu, Xiaojuan; Xiong, Guihong; Qiu, Ping; Du, Zhenguo; Kormelink, Richard; Zheng, Luping; Zhang, Jie; Ding, Xinlun; Yang, Liang; Zhang, Songbai; Wu, Zujian
Source Virology 496 (2016). - ISSN 0042-6822 - p. 287 - 298.
DOI https://doi.org/10.1016/j.virol.2016.06.018
Department(s) Laboratory of Virology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Cap-snatching - Co-infection - Plant-infecting reovirus - Prime-and-realign - Tenuivirus
Abstract

Two tenuiviruses Rice stripe virus (RSV) and Rice grassy stunt virus (RGSV) were found to co-infect rice with the same reovirus Rice ragged stunt virus (RRSV). During the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-RNA leaders from the RRSV. A total of 245 and 102 RRSV-RGSV and RRSV-RSV chimeric mRNA clones, respectively, were sequenced. An analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped leader RNAs having a 3' end complementary to the viral template are preferred and upon base pairing the leaders prime processive transcription directly or after one to several cycles of priming and realignment (repetitive prime-and-realign). Interestingly, RSV appeared to have a higher tendency to use repetitive prime-and-realign than RGSV even with the same leader derived from the same RRSV RNA. Combining with relevant data reported previously, this points towards an intrinsic feature of RSV.

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