|Title||Inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of Rice stripe virus|
|Author(s)||Liu, Xiaojuan; Xiong, Guihong; Qiu, Ping; Du, Zhenguo; Kormelink, Richard; Zheng, Luping; Zhang, Jie; Ding, Xinlun; Yang, Liang; Zhang, Songbai; Wu, Zujian|
|Source||Virology 496 (2016). - ISSN 0042-6822 - p. 287 - 298.|
Laboratory of Virology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Cap-snatching - Co-infection - Plant-infecting reovirus - Prime-and-realign - Tenuivirus|
Two tenuiviruses Rice stripe virus (RSV) and Rice grassy stunt virus (RGSV) were found to co-infect rice with the same reovirus Rice ragged stunt virus (RRSV). During the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-RNA leaders from the RRSV. A total of 245 and 102 RRSV-RGSV and RRSV-RSV chimeric mRNA clones, respectively, were sequenced. An analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped leader RNAs having a 3' end complementary to the viral template are preferred and upon base pairing the leaders prime processive transcription directly or after one to several cycles of priming and realignment (repetitive prime-and-realign). Interestingly, RSV appeared to have a higher tendency to use repetitive prime-and-realign than RGSV even with the same leader derived from the same RRSV RNA. Combining with relevant data reported previously, this points towards an intrinsic feature of RSV.