Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 506743
Title Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses
Author(s) Coolen, Silvia; Proietti, Silvia; Hickman, Richard; Davila Olivas, Nelson H.; Huang, Pingping; Verk, Marcel C. van; Pelt, Johan A. van; Wittenberg, Alexander H.J.; Vos, Martin de; Prins, Marcel; Loon, Joop J.A. van; Aarts, Mark G.M.; Dicke, Marcel; Pieterse, Corné M.J.; Wees, Saskia C.M. van
Source The Plant Journal 86 (2016)3. - ISSN 0960-7412 - p. 249 - 267.
DOI https://doi.org/10.1111/tpj.13167
Department(s) Laboratory of Entomology
Groep KoornneefGroep Koornneef
EPS
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Arabidopsis thaliana - Botrytis cinerea - combinatorial plant stress - drought stress - gene regulatory network - Pieris rapae - plant hormones - RNA-Seq - transcript profiling - 016-3950
Abstract

In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how plants cope with multi-stress conditions, we analyzed the dynamics of whole-transcriptome profiles of Arabidopsis thaliana exposed to six sequential double stresses inflicted by combinations of: (i) infection by the necrotrophic fungus Botrytis cinerea, (ii) herbivory by chewing larvae of Pieris rapae, and (iii) drought stress. Each of these stresses induced specific expression profiles over time, in which one-third of all differentially expressed genes was shared by at least two single stresses. Of these, 394 genes were differentially expressed during all three stress conditions, albeit often in opposite directions. When two stresses were applied in sequence, plants displayed transcriptome profiles that were very similar to the second stress, irrespective of the nature of the first stress. Nevertheless, significant first-stress signatures could be identified in the sequential stress profiles. Bioinformatic analysis of the dynamics of co-expressed gene clusters highlighted specific clusters and biological processes of which the timing of activation or repression was altered by a prior stress. The first-stress signatures in second stress transcriptional profiles were remarkably often related to responses to phytohormones, strengthening the notion that hormones are global modulators of interactions between different types of stress. Because prior stresses can affect the level of tolerance against a subsequent stress (e.g. prior herbivory strongly affected resistance to B. cinerea), the first-stress signatures can provide important leads for the identification of molecular players that are decisive in the interactions between stress response pathways.

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