Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 507130
Title Repeated nitrogen starvation doesn't affect lipid productivity of Chlorococcum littorale
Author(s) Dominguez Teles, Iago; Kleinegris, Dorinde M.M.; Wijffels, René H.; Barbosa, Maria J.
Source Bioresource Technology 219 (2016). - ISSN 0960-8524 - p. 576 - 582.
Department(s) Bioprocess Engineering
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Adaptive laboratory evolution (ALE) - Chlorococcum littorale - Microalgae - Nitrogen-starvation - Repeated-batch

In the present work we wanted to know what happens during time to biomass and lipid productivities of Chlorococcum littorale repeatedly subjected to N-starvation. Experiments were done using repeated cycles of batch-wise N run-out (after 2 days N = 0). Two different cycles were used: repeated short-starvation (6 days of N = 0) over a total period of 72 days and repeated long-starvation (13 days of N = 0) over a total period of 75 days. Batches (using fresh inocula) were done separately as control. Shorter and longer periods of starvation showed no differences in biomass productivities and PSII quantum yield evolution. The repeated short-starvation-batches showed the same lipid productivities as the control short-starvation batches. Most importantly, the biomass lipid content was the same between control and repeated-batches. Altogether, the results point to C. littorale as a resilient and stable strain, with potential to be used under semi continuous cultivation.

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