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Record number 507274
Title Proteomic analysis of the hydrogen and carbon monoxide metabolism of methanothermobacter marburgensis
Author(s) Diender, Martijn; Afonso Goncalves Pereira, Ricardo; Wessels, Hans J.C.T.; Stams, Fons; Machado de Sousa, Diana
Source Frontiers in Microbiology 7 (2016). - ISSN 1664-302X
DOI https://doi.org/10.3389/fmicb.2016.01049
Department(s) Microbiological Laboratory
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) CODH - Methanogenesis - Methanothermobacter thermoautotrophicus - Reductive acetyl-CoA pathway - Syngas
Abstract

Hydrogenotrophic methanogenic archaea are efficient H2 utilizers, but only a few are known to be able to utilize CO. Methanothermobacter thermoautotrophicus is one of the hydrogenotrophic methanogens able to grow on CO, albeit about 100 times slower than on H2 + CO2. In this study, we show that the hydrogenotrophic methanogen Methanothermobacter marburgensis, is able to perform methanogenic growth on H2/CO2/CO and on CO as a sole substrate. To gain further insight in its carboxydotrophic metabolism, the proteome of M. marburgensis, grown on H2/CO2 and H2/CO2/CO, was analyzed. Cultures grown with H2/CO2/CO showed relative higher abundance of enzymes involved in the reductive acetyl-CoA pathway and proteins involved in redox metabolism. The data suggest that the strong reducing capacity of CO negatively affects hydrogenotrophic methanogenesis, making growth on CO as a sole substrate difficult for this type of methanogens. M. marburgensis appears to partly deal with this by up-regulating co-factor regenerating reactions and activating additional pathways allowing for formation of other products, like acetate.

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