|Title||Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T|
|Author(s)||Bosma, Elleke F.; Koehorst, Jasper J.; Hijum, Sacha A.F.T. van; Renckens, Bernadet; Vriesendorp, Bastienne; Weijer, Tom van de; Schaap, Peter J.; Vos, Willem M. de; Oost, John van der; Kranenburg, Richard van|
|Source||Standards in Genomic Sciences 11 (2016). - ISSN 1944-3277 - 11 p.|
Systems and Synthetic Biology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Bacillus smithii - Biotechnology - Genome sequence - Lactic acid - Thermophile - Thermophilic bacillus|
Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.