Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 507683
Title Comprehensive DNA Methylation and Gene Expression Profiling in Differentiating Human Adipocytes
Author(s) Dungen, M.W. van den; Murk, Albertinka J.; Kok, D.E.G.; Steegenga, W.T.
Source Journal of Cellular Biochemistry 117 (2016)12. - ISSN 0730-2312 - p. 2707 - 2718.
DOI http://dx.doi.org/10.1002/jcb.25568
Department(s) Sub-department of Environmental Technology
Marine Animal Ecology
Chair Nutrition and Disease
VLAG
Chair Nutrition Metabolism and Genomics
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) DNA METHYLATION - ILLUMINA 450K BEADCHIP - adipocyte differentiation - HUMAN MESENCHYMAL STEM CELLS - GENE EXPRESSION
Abstract Insight into the processes controlling adipogenesis is important in the battle against the obesity epidemic and its related disorders. The transcriptional regulatory cascade involved in adipocyte differentiation has been extensively studied, however, the mechanisms driving the transcription activation are still poorly understood. In this study, we explored the involvement of DNA methylation in transcriptional regulation during adipocyte differentiation of primary human mesenchymal stem cells (hMSCs). Genome-wide changes in DNA methylation were measured using the Illumina 450K BeadChip. In addition, expression of 84 adipogenic genes was determined, of which 43 genes showed significant expression changes during the differentiation process. Among these 43 differentially expressed genes, differentially methylated regions (DMRs) were detected in only three genes. By comparing genome-wide DNA methylation profiles in undifferentiated and differentiated adipocytes 793 significant DMRs were detected. Pathway analysis revealed the adipogenesis pathway as the most statistically significant, although only a small number of genes were differentially methylated. Genome-wide DNA methylation changes for single probes were most often located in intergenic regions, and underrepresented close to the transcription start site. In conclusion, DNA methylation remained relatively stable during adipocyte differentiation, implying that changes in DNA methylation are not the underlying mechanism regulating gene expression during adipocyte differentiation.
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