Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 507692
Title Different responses of Caco-2 and MCF-7 cells to silver nanoparticles are based on highly similar mechanisms of action
Author(s) Zande, Meike van der; Undas, Anna K.; Kramer, Evelien; Monopoli, Marco P.; Peters, Ruud J.; Garry, David; Antunes Fernandes, Elsa C.; Hendriksen, Peter J.; Marvin, Hans J.P.; Peijnenburg, Ad A.; Bouwmeester, Hans
Source Nanotoxicology 10 (2016)10. - ISSN 1743-5390 - p. 1431 - 1441.
DOI http://dx.doi.org/10.1080/17435390.2016.1225132
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
RIKILT - Business unit Contaminants & Toxins
Food Quality and Design
VLAG
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Caco-2 - in vitro - MCF-7 - silver nanoparticles - toxicogenomics
Abstract

The mode of action of silver nanoparticles (AgNPs) is suggested to be exerted through both Ag+ and AgNP dependent mechanisms. Ingestion is one of the major NP exposure routes, and potential effects are often studied using Caco-2 cells, a well-established model for the gut epithelium. MCF-7 cells are epithelial breast cancer cells with extensive well-characterized toxicogenomics profiles. In the present study, we aimed to gain a deeper understanding of the cellular molecular responses in Caco-2 and MCF-7 cells after AgNP exposure in order to evaluate whether epithelial cells derived from different tissues demonstrated similar responses. These insights could possibly reduce the size of cell panels for NP hazard identification screening purposes. AgNPs of 20, 30, 60, and 110 nm, and AgNO3 were exposed for 6 h and 24 h. AgNPs were shown to be taken up and dissolve intracellularly. Compared with MCF-7 cells, Caco-2 cells showed a higher sensitivity to AgNPs, slower gene expression kinetics and absence of NP size-dependent responses. However, on a molecular level, no significant differences were observed between the two cell types. Transcriptomic analysis showed that Ag(NP) exposure caused (oxidative) stress responses, possibly leading to cell death in both cell lines. There was no indication for effects specifically induced by AgNPs. Responses to AgNPs appeared to be induced by silver ions released from the AgNPs. In conclusion, differences in mRNA responses to AgNPs between Caco-2 and MCF-7 cells were mainly related to timing and magnitude, but not to a different underlying mechanism.

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