Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 508098
Title Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation : Analysis of 672 individual repeated measurements
Author(s) Mil, Anke C.C.M. van; Greyling, Arno; Zock, Peter L.; Geleijnse, Marianne; Hopman, Maria T.; Mensink, Ronald P.; Reesink, Koen D.; Green, Daniel J.; Ghiadoni, Lorenzo; Thijssen, Dick H.
Source Journal of Hypertension 34 (2016)9. - ISSN 0263-6352 - p. 1738 - 1745.
DOI https://doi.org/10.1097/HJH.0000000000001012
Department(s) Chair Nutrition and Disease
VLAG
Human Nutrition (HNE)
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Doppler - endothelial function - flow-mediated dilation - reproducibility - ultrasonography
Abstract

Objectives: Brachial artery flow-mediated dilation (FMD) is a popular technique to examine endothelial function in humans. Identifying volunteer and methodological factors related to variation in FMD is important to improve measurement accuracy and applicability. Methods: Volunteer-related and methodology-related parameters were collected in 672 volunteers from eight affiliated centres worldwide who underwent repeated measures of FMD. All centres adopted contemporary expert-consensus guidelines for FMD assessment. After calculating the coefficient of variation (%) of the FMD for each individual, we constructed quartiles (n = 168 per quartile). Based on two regression models (volunteer-related factors and methodology-related factors), statistically significant components of these two models were added to a final regression model (calculated as β-coefficient and R 2). This allowed us to identify factors that independently contributed to the variation in FMD%. Results: Median coefficient of variation was 17.5%, with healthy volunteers demonstrating a coefficient of variation 9.3%. Regression models revealed age (β = 0.248, P <0.001), hypertension (β = 0.104, P <0.001), dyslipidemia (β = 0.331, P <0.001), time between measurements (β = 0.318, P <0.001), lab experience (β = -0.133, P <0.001) and baseline FMD% (β = 0.082, P <0.05) as contributors to the coefficient of variation. After including all significant factors in the final model, we found that time between measurements, hypertension, baseline FMD% and lab experience with FMD independently predicted brachial artery variability (total R2 = 0.202). Conclusion: Although FMD% showed good reproducibility, larger variation was observed in conditions with longer time between measurements, hypertension, less experience and lower baseline FMD%. Accounting for these factors may improve FMD% variability.

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