Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 508125
Title Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
Author(s) Ippolito, Sebastian D'; Vankova, Radomira; Joosten, Matthieu H.A.J.; Casalongué, Claudia A.; Fiol, Diego F.
Source Plant Science 253 (2016). - ISSN 0168-9452 - p. 31 - 39.
DOI https://doi.org/10.1016/j.plantsci.2016.09.008
Department(s) Laboratory of Phytopathology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Auxin - Biotic stress - Cladosporium fulvum - Indole-3-acetic acid amido hydrolases - Nicotiana benthamiana - Phytophthora infestans - Solanum lycopersicum
Abstract

In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemi-biotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.

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