Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 508637
Title Extraction of Proteins with ABS
Author(s) Desai, R.K.; Streefland, M.; Wijffels, R.H.; Eppink, M.H.M.
Source In: Ionic-liquid-based aqueous biphasic systems: Fundamentals and applications / Freire, Mara G., Springer Verlag (Green Chemistry and Sustainable Technology ) - ISBN 9783662528730 - p. 123 - 134.
Department(s) Bioprocess Engineering
Publication type Peer reviewed book chapter
Publication year 2016
Abstract Over the past years, there has been an increasing trend in research on the extraction and purification of proteins using aqueous biphasic systems (ABS) formed by polymers, e.g., polyethylene glycol (PEG). In general, when dealing with protein purification processes, it is essential to maintain their native structure and functional stability. In this context, ABS, liquid-liquid systems where both phases are water-rich, provide a biocompatible medium for such attempts. More recently, it was shown that the versatility offered by ABS is further enhanced by the introduction of ionic liquids (ILs) as alternative phase-forming components. This chapter describes and highlights the current progress on the field of protein extraction and purification using IL-based ABS. The general approach for protein extraction using IL-based ABS and factors influencing the partitioning are discussed. In addition, the challenges to overcome the use of IL-based ABS for protein extraction are also presented.
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