Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 508782
Title Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices
Author(s) Witkamp, Renger F.; Balvers, Michiel
Source In: Endocannabinoid signaling: Methods and Protocols / Maccarrone, Mauro, Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781493935376 - p. 27 - 40.
DOI https://doi.org/10.1007/978-1-4939-3539-0_4
Department(s) Chair Nutrition and Pharmacology (HNE)
VLAG
Publication type Peer reviewed book chapter
Publication year 2016
Keyword(s) Docosahexaenoylethanolamide - Eicosapentaenoylethanolamide - Endocannabinoids - LC-MS - N-3 fatty acids - Solid phase extraction
Abstract

The adequate quantification of endocannabinoids can be complex due to their low endogenous levels and structural diversity. Therefore, advanced analytical approaches, such as LC-MS, are used to measure endocannabinoids in plasma, tissues, and other matrices. Recent work has shown that endocannabinoids that are synthesized from n-3 fatty acids, such as docosahexaenoylethanolamide (DHEA) and eicosapentaenoylethanolamide (EPEA), have anti-inflammatory and anti-tumorigenic properties and stimulate synapse formation in neurites. Here, an LC-MS based method for the quantification of n-3 endocannabinoids DHEA and EPEA which is also suited to measure a wider spectrum of endocannabinoids is described. The chapter contains a step-by-step protocol for the analysis of n-3 endocannabinoids in plasma, including sample collection and solid phase extraction, LC-MS analysis, and data processing. Modifications to the protocol that allow quantifying n-3 endocannabinoids in tissues and cell culture media will also be discussed. Finally, conditions that alter endocannabinoid concentrations are briefly discussed.

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