|Title||Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability|
|Author(s)||Kuijk, S.J.A. van; Rio, José C. del; Rencoret, Jorge; Gutiérrez, Ana; Sonnenberg, A.S.M.; Baars, J.J.P.; Hendriks, W.H.; Cone, J.W.|
|Source||Journal of Animal Science and Biotechnology 7 (2016). - ISSN 1674-9782 - 14 p.|
WUR PB Paddenstoelen
|Publication type||Refereed Article in a scientific journal|
|Abstract||Background: The present work investigated the influence of lignin content and composition in the fungal
treatment of lignocellulosic biomass in order to improve rumen degradability. Wheat straw and wood chips,
differing in lignin composition, were treated with Lentinula edodes for 0, 2, 4, 8 and 12 wk and the changes
occurring during fungal degradation were analyzed using pyrolysis-gas chromatography-mass spectrometry
and detergent fiber analysis.
Results: L. edodes preferentially degraded lignin, with only limited cellulose degradation, in wheat straw and
wood chips, leaving a substrate enriched in cellulose. Syringyl (S)-lignin units were preferentially degraded than
guaiacyl (G)-lignin units, resulting in a decreased S/G ratio. A decreasing S/G ratio (wheat straw: r = −0.72, wood
chips: r = −0.75) and selective lignin degradation (wheat straw: r = −0.69, wood chips: r = −0.88) were correlated
with in vitro gas production (IVGP), a good indicator for rumen degradability.
Conclusions: L. edodes treatment increased the IVGP of wheat straw and wood chips. Effects on IVGP were
similar for wheat straw and wood chips indicating that lignin content and 3D-structure of cell walls influence
in vitro rumen degradability more than lignin composition.
Keywords: Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Py-GC/MS