Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 509484
Title Thermodynamic driving force of hydrogen on rumen microbial metabolism : A theoretical investigation
Author(s) Lingen, Henk J. Van; Plugge, Caroline M.; Fadel, James G.; Kebreab, Ermias; Bannink, André; Dijkstra, Jan
Source PLoS ONE 11 (2016)10. - ISSN 1932-6203
DOI https://doi.org/10.1371/journal.pone.0161362
Department(s) Animal Nutrition
Microbiological Laboratory
WIMEK
LR - Animal Nutrition
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract

Hydrogen is a key product of rumen fermentation and has been suggested to thermodynamically control the production of the various volatile fatty acids (VFA). Previous studies, however, have not accounted for the fact that only thermodynamic near-equilibrium conditions control the magnitude of reaction rate. Furthermore, the role of NAD, which is affected by hydrogen partial pressure (PH 2), has often not been considered. The aim of this study was to quantify the control of PH 2 on reaction rates of specific fermentation pathways, methanogenesis and NADH oxidation in rumen microbes. The control of PH 2 was quantified using the thermodynamic potential factor (FT), which is a dimensionless factor that corrects a predicted kinetic reaction rate for the thermodynamic control exerted. Unity FT was calculated for all glucose fermentation pathways considered, indicating no inhibition of PH 2 on the production of a specific type of VFA (e.g., acetate, propionate and butyrate) in the rumen. For NADH oxidation without ferredoxin oxidation, increasing PH 2 within the rumen physiological range decreased FT from unity to zero for different NAD+ to NADH ratios and pH of 6.2 and 7.0, which indicates thermodynamic control of PH 2. For NADH oxidation with ferredoxin oxidation, increasing PH 2 within the rumen physiological range decreased FT from unity at pH of 7.0 only. For the acetate to propionate conversion, FT increased from 0.65 to unity with increasing PH 2, which indicates thermodynamic control. For propionate to acetate and butyrate to acetate conversions, FT decreased to zero below the rumen range of PH 2, indicating full thermodynamic suppression. For methanogenesis by archaea without cytochromes, FT differed from unity only below the rumen range of PH 2, indicating no thermodynamic control. This theoretical investigation shows that thermodynamic control of PH 2 on individual VFA produced and associated yield of hydrogen and methane cannot be explained without considering NADH oxidation.

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