Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 510053
Title Detection of peanut allergen in human blood after consumption of peanuts is skewed by endogenous immunoglobulins
Author(s) Janssen Duijghuijsen, L.M.; Wichers, H.J.; Norren, K. van; Keijer, J.; Baumert, J.L.; Jong, Govardus A.H. De; Witkamp, R.F.; Koppelman, S.J.
Source Journal of Immunological Methods 440 (2017). - ISSN 0022-1759 - p. 52 - 57.
Department(s) FBR Consumer Science & Health
Chair Nutrition and Pharmacology (HNE)
Human and Animal Physiology
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract Some studies have suggested that allergens may appear in the circulation after ingestion of allergenic food
sources. The reported levels of allergen in serum, however, are low, and conclusions between studies differ.
Here, we investigated factors that determine the detection of allergens in serum after consumption of peanuts.
Ten healthy volunteers ingested 100 g of light-roasted peanuts. Serumsampleswere taken at regular intervals for
six hours. A double monoclonal sandwich ELISAwas used to analyse the presence and quantity of the major peanut
allergen Ara h 6 in serum.
In 4 out of 10 subjects, no Ara h 6 could be detected. Purified Ara h 6 thatwas digested in vitrowas still reactive in
the ELISA, rejecting the possibility that digestion leads to small peptides that could not be detected. Spiking of purified
Ara h 6 in baseline serum showed that the pre-ingestion serum of these four subjects partially prevented
Ara h 6 to react in the ELISA, with a reduction of reactivity of up to 3 orders of magnitude or more. Pre-ingestion
serum of the other six subjects did not show such an effect. The reduction of reactivity of Ara h 6 coincided with
high titres of IgG and IgG4, and removal of IgG from pre-ingestion serum abolished this effect completely, indicating
that IgG and IgG4 inhibited the reactivity of Ara h6 in the ELISA.
Weconclude that some individuals have IgG and IgG4 against food allergens in their blood, which interfereswith
detection of such food allergens in serum. Because this effect does not occur for each individual, the possibility of
such interference should be taken into considerationwhen interpreting immunochemical studies on the absorption
of food allergens in serum.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.