Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510089
Title Bidirectional sRNA-trafficking and RNA-based plant protection against Botrytis cinerea and other pathogens that utilize small RNA effectors
Author(s) Wang, M.; Weiberg, A.; Lin, F.; Thomma, B.P.H.J.; Huang, H.; Jin, H.
Event XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, 2016-07-17/2016-07-21
Department(s) Laboratory of Phytopathology
EPS
Publication type Abstract in scientific journal or proceedings
Publication year 2016
Abstract The aggressive fungal pathogen Botrytis cinerea causes severe crop losses worldwide. We have discovered that B. cinerea delivers small RNAs (sRNAs) into plant cells to silence host immunity genes. Such sRNA effectors are mainly produced from retrotransposons by B. cinerea Dicer-like (Bc-DCL) proteins. Here we show that expressing sRNAs that target Bc-DCL1 and Bc-DCL2 in Arabidopsis or Solanum lycopersium (tomato) silences Bc-DCL genes and attenuates fungal pathogenicity and growth. This suggests that sRNAs can move from the hosts to the fungus; thus, sRNA trafficking between B. cinerea and its hosts is bidirectional. We demonstrate that Verticillum dahliae, a fungal pathogen that causes wilt disease, also uses sRNA effectors for its virulence. Plants expressing sRNAs that target DCLs of B. cinerea and V. dahliae, show reduced susceptibility to both pathogens. Furthermore, B. cinerea could take up external sRNAs and dsRNAs from the environment. Applying Bc-DCL-targeting sRNAs or the double-stranded RNA (dsRNA) precursors on the surface of fruits, vegetables, or flowers significantly inhibits gray mold disease. These results demonstrate an effective RNAi-based strategy for controlling multiple diseases, which could potentially replace toxic fungicides and also circumvents the need for transgenic plants.
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