Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 510092
Title Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript
Author(s) Damme, M.M.A. van; Berg, G. van den; Faino, L.; Thomma, B.P.H.J.
Event XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, 2016-07-17/2016-07-21
Department(s) Laboratory of Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2016
Abstract RNA silencing is a eukaryotic mechanism that exploits small non-coding RNAs (small-RNAs) to regulate gene expression in a sequence-specific manner in many cellular processes, including innate immunity. The plant immune system is a known target of RNA silencing by several pathogens, e.g. viruses and bacteria. The fungus Verticillium dahliae is the causal agent of Verticillium wilt disease. Data from our laboratory indicate that the Arabidopsis thaliana RNA silencing machinery plays a critical role during Verticillium wilt disease (Ellendorff et al., 2009). By combining small-RNA and transcript profiling we identified small-RNAs and their reciprocal target transcripts in V. dahliae-infected A. thaliana plants. Accumulation of a particular A. thaliana miRNA during the interaction, and repression of its reciprocal targeted transcript, causes enhanced resistance to Verticillium wilt disease. We show that A. thaliana T-DNA insertion mutants that lack the target transcript display enhanced resistance to Verticillium wilt disease. Mature miRNAs are generated through multiple processing steps from primary transcripts (pri-miRNA or MIRNA gene) that contain imperfect foldback structures. We are currently analyzing if transgenic A. thaliana lines that overexpress the MIRNA gene also display enhanced resistance to Verticillium wilt disease.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.