Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510288
Title In vivo identification of plant protein complexes using IP-MS/MS
Author(s) Wendrich, Jos R.; Boeren, Sjef; Möller, Barbara; Weijers, Dolf; Rybel, Bert de
Source In: Methods in Molecular Biology / Kleine-Vehn, Jürgen, Sauer, Michael, Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781493964673 - p. 147 - 158.
DOI http://dx.doi.org/10.1007/978-1-4939-6469-7_14
Department(s) Biochemistry
EPS
Publication type Peer reviewed book chapter
Publication year 2017
Keyword(s) Arabidopsis - GFP - Immunoprecipitation - Protein complex identification - Protein–protein interaction - Tandem mass spectrometry
Abstract

Individual proteins often function as part of a protein complex. The identification of interacting proteins is therefore vital to understand the biological role and function of the studied protein. Here we describe a method for the in vivo identification of nuclear, cytoplasmic, and membrane-associated protein complexes from plant tissues using a strategy of immunoprecipitation followed by tandem mass spectrometry. By performing quantitative mass spectrometry measurements on biological triplicates, relative abundance of proteins in GFP-tagged complexes compared to background controls can be statistically evaluated to identify high-confidence interactors. We detail the entire workflow of this approach.

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