Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510289
Title FRET-FLIM for visualizing and quantifying protein interactions in live plant cells
Author(s) Rios, Alejandra Freire; Radoeva, Tatyana; Rybel, Bert de; Weijers, Dolf; Borst, Janwillem
Source In: Methods in Molecular Biology / Kleine-Vehn, Jürgen, Sauer, Michael, Humana Press Inc. (Methods in Molecular Biology ) - ISBN 9781493964673 - p. 135 - 146.
DOI https://doi.org/10.1007/978-1-4939-6469-7_13
Department(s) Biochemistry
EPS
Publication type Peer reviewed book chapter
Publication year 2017
Keyword(s) FLIM - Fluorescent proteins - FRET - Protein interactions
Abstract

Proteins are the workhorses that control most biological processes in living cells. Although proteins can accomplish their functions independently, the vast majority of functions require proteins to interact with other proteins or biomacromolecules. Protein interactions can be investigated through biochemical assays such as co-immunoprecipitation (co-IP) or Western blot analysis, but such assays lack spatial information. Here we describe a well-developed imaging method, Förster resonance energy transfer (FRET) analyzed by fluorescence lifetime imaging microscopy (FLIM), that can be used to visualize protein interactions with both spatial and temporal resolution in live cells. We demonstrate its use in plant developmental research by visualizing in vivo dimerization of AUXIN RESPONSE FACTOR (ARF) proteins, mediating auxin responses.

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