Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510357
Title Abundance, Activity and Community Structure of Denitrifiers in Drainage Ditches in Relation to Sediment Characteristics, Vegetation and Land-Use
Author(s) Veraart, Annelies J.; Rocha Dimitrov, Mauricio; Schrier-Uijl, Arina P.; Smidt, Hauke; Klein, Jeroen J.M. de
Source Ecosystems 20 (2017)5. - ISSN 1432-9840 - p. 928 - 943.
DOI http://dx.doi.org/10.1007/s10021-016-0083-y
Department(s) Microbiological Laboratory
VLAG
WIMEK
Aquatic Ecology and Water Quality Management
Publication type Refereed Article in a scientific journal
Publication year 2017
Keyword(s) agroecosystem - denitrification - DGGE - ecosystem functioning - macrophytes - nitrogen - qPCR - wetlands
Abstract Drainage ditches are ubiquitous yet understudied features of the agricultural landscape. Nitrogen pollution disrupts the nutrient balance of drainage ditch ecosystems, as well as the waterbodies in which they drain. Denitrification can help ameliorate the impact of N-fertilization by converting reactive nitrogen into dinitrogen gas. However, factors affecting denitrification in drainage ditches are still poorly understood. In this study, we tested how within-ditch and regional environmental conditions affect denitrifier activity, abundance, and community structure, to understand controls on denitrification at multiple scales. To this end, we quantified in situ denitrification rates and denitrifier abundance in 13 drainage ditches characterized by different types of sediment, vegetation and land-use. We determined how denitrification rates relate to denitrifier abundance and community structure, using the presence of nirS, nirK and nosZ genes as a proxy. Denitrification rates varied widely between the ditches, ranging from 0.006 to 24 mmol N m−2 h−1. Ditches covered by duckweed, which contained high nitrate concentrations and had fine, sandy sediments, were denitrification hotspots. We found highest rates in ditches next to arable land, followed by those in grasslands; lowest rates were observed in peatlands and nature reserves. Denitrification correlated to nitrate concentrations, but not to nirK, nirS and nosZ abundance, whereas denitrifier-gene abundance correlated to organic matter content of the sediment, but not to nitrate concentrations. Our results show a mismatch in denitrification regulators at its different organizational scales. Denitrifier abundance is mostly regulated at within-ditch scales, whereas N-loads, regulated by landscape factors, are most important determinants of instantaneous denitrification rates.
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