Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510372
Title Biorefinery of microalgal soluble proteins by sequential processing and membrane filtration
Author(s) Safi, C.; Olivieri, G.; Pina Campos, Rui; Engelen-Smit, N.; Mulder, W.J.; Broek, L.A.M. van den; Sijtsma, L.
Source Bioresource Technology 225 (2017). - ISSN 0960-8524 - p. 151 - 158.
DOI https://doi.org/10.1016/j.biortech.2016.11.068
Department(s) Bioprocess Engineering
FBR BP Biorefinery & Sustainable Value Chains
VLAG
FBR Sustainable Chemistry & Technology
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2017
Keyword(s) Biorefinery - Cell disruption - Fractionation - Proteins - Ultrafiltration
Abstract

A mild biorefinery process was investigated on the microalga Nannochloropsis gaditana, to obtain an enriched fraction of water soluble proteins free from chlorophyll. After harvesting, a 100 g.L−1 solution of cells was first subjected to cell disruption by either high-pressure homogenization (HPH) or enzymatic treatment (ENZ). HPH resulted in a larger release of proteins (49%) in the aqueous phase compared to the Alcalase incubation (35%). In both cases, an ultrafiltration/diafiltration (UF/DF) was then performed on the supernatant obtained from cell disruption by testing different membrane cut-off (1000 kDa, 500 kDa and 300 kDa). After optimising the process conditions, the combination of ENZ → UF/DF ended in a larger overall yield of water soluble proteins (24.8%) in the permeate compared to the combination of HPH → UF/DF (17.4%). A gel polarization model was implemented to assess the maximum achievable concentration factor during ultrafiltration and the mass transfer coefficient related to the theoretical permeation flux rate.

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