Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510711
Title Selection of chemically defined media for CHO cell fed-batch culture processes
Author(s) Pan, X.; Streefland, M.; Dalm, C.; Wijffels, R.H.; Martens, D.E.
Source Cytotechnology 69 (2017)1. - p. 39 - 56.
DOI https://doi.org/10.1007/s10616-016-0036-5
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract Two CHO cell clones derived from the same parental CHOBC cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures.
Higher amino acid feeding did not always lead to igher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and roduction rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B.
The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary
phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B).
The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.
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