Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510713
Title Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates
Author(s) Postma, P.R.; Suarez Garcia, E.; Safi, Carl; Yonathan, K.; Olivieri, G.; Barbosa, M.J.; Wijffels, R.H.; Eppink, M.H.M.
Source Bioresource Technology 224 (2017). - ISSN 0960-8524 - p. 670 - 679.
DOI http://dx.doi.org/10.1016/j.biortech.2016.11.071
Department(s) Bioprocess Engineering
FBR BP Biorefinery & Sustainable Value Chains
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract The disintegration of three industry relevant algae (Chlorella vulgaris, Neochloris oleoabundans and Tetraselmis suecica) was studied in a lab scale bead mill at different bead sizes (0.3–1 mm). Cell disintegration, proteins and carbohydrates released into the water phase followed a first order kinetics. The process
is selective towards proteins over carbohydrates during early stages of milling. In general, smaller beads led to higher kinetic rates, with a minimum specific energy consumption of 60.47 kWh kgDW1 for 0.3 mm beads. After analysis of the stress parameters (stress number and stress intensity), it appears that
optimal disintegration and energy usage for all strains occurs in the 0.3–0.4 mm range. During the course of bead milling, the native structure of the marker protein Rubisco was retained, confirming the mildness f the disruption process.
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