Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 510841
Title Effect of humic acid on anaerobic digestion of cellulose and xylan in completely stirred tank reactors : inhibitory effect, mitigation of the inhibition and the dynamics of the microbial communities
Author(s) Azman, Samet; Khadem, Ahmad F.; Plugge, Caroline M.; Stams, Fons; Bec, Sabina; Zeeman, Grietje
Source Applied Microbiology and Biotechnology 101 (2017)2. - ISSN 0175-7598 - p. 889 - 901.
DOI https://doi.org/10.1007/s00253-016-8010-x
Department(s) Microbiological Laboratory
WIMEK
VLAG
Sub-department of Environmental Technology
Publication type Refereed Article in a scientific journal
Publication year 2017
Keyword(s) Calcium - Hydrolysis - Hydrolytic enzymes - Inhibition - Microbial community analyses - Mitigation
Abstract

Inhibition effect of humic acid (HA) on anaerobic digestion of cellulose and xylan and the mitigation potential of the inhibition were evaluated in controlled fed batch reactors at 30 °C and a hydraulic retention time (HRT) of 20 days. Reactor performances were evaluated by biogas production and metabolite measurements for 220 days. Microbial population dynamics of the reactors were monitored with next-generation 16S rRNA gene sequencing at nine different sampling times. Our results showed that increasing levels of HA inhibited the hydrolysis efficiency of the digestion by 40% and concomitantly reduced the methane yield. Addition of hydrolytic enzymes helped to reverse the negative effects of HA, whereas calcium addition did not reverse HA inhibition. Microbiological analyses showed that the relative abundance of hydrolytic/fermentative bacterial groups such as Clostridiales, Bacteroidales and Anaerolineales was significantly lowered by the presence of HA. HA also affected the archaeal populations. Mostly hydrogenotrophic methanogens were negatively affected by HA. The relative abundance of Methanobacteriaceae, Methanomicrobiales-WCHA208 and Unassigned Thermoplasmata WCHA1-57 were negatively affected by the presence of HA, whereas Methanosaetacea was not affected.

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