Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 511210
Title Expression of an (engineered) 4,6-α-glucanotransferase in potato results in changes in starch characteristics
Author(s) Xu, Xuan; Dechesne, Annemarie; Visser, Richard G.F.; Trindade, Luisa M.
Source PLoS One 11 (2016)12. - ISSN 1932-6203
DOI http://dx.doi.org/10.1371/journal.pone.0166981
Department(s) Laboratory of Plant Breeding
WUR PB Biobased Economy
PE&RC
EPS
PBR Biobased Economy
Publication type Refereed Article in a scientific journal
Publication year 2016
Abstract

Starch structure strongly influences starch physicochemical properties, determining the end uses of starch in various applications. To produce starches with novel structure and exploit the mechanism of starch granule formation, an (engineered) 4, 6-α-glucanotransferase (GTFB) from Lactobacillus reuteri 121 was introduced into two potato genetic backgrounds: amylose-containing line Kardal and amylose-free mutant amf. The resulting starches showed severe changes in granule morphology regardless of genetic backgrounds. Modified starches from amf background exhibited a significant increase in granule size and starch phosphate content relative to the control, while starches from Kardal background displayed a higher digestibility, but did not show changes in granule size and phosphate content. Transcriptome analysis revealed the existence of a mechanism to restore the regular packing of double helices in starch granules, which possibly resulted in the removal of novel glucose chains potentially introduced by the (engineered) GTFB. This amendment mechanics would also explain the difficulties to detect alterations in starch fine structure in the transgenic lines.

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