Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 520505
Title Temperature-dependent alternative splicing of FLM controls flowering in Arabidopsis thaliana
Author(s) Posé, D.; Verhage, D.S.L.; Yant, L.; Mathieu, J.; Angenent, G.C.; Immink, G.H.; Schmid, M.
Department(s) PRI BIOS Plant Development Systems
Laboratory of Molecular Biology
EPS
Publication type Dataset
Publication year 2016
Keyword(s) Arabidopsis thaliana - GSE48082 - PRJNA208886
Abstract How plants control the transition to flowering in response to ambient temperature is only beginning to be understood. In Arabidopsis thaliana, the MADS-box transcription factor genes FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) have key roles in this process. FLM is subject to temperature-dependent alternative splicing, producing two splice variants, FLM-β and FLM-δ, which compete for interaction with the floral repressor SVP. The SVP/FLM-β complex is predominately formed at low temperatures and prevents precocious flowering. In contrast, the competing SVP FLM-δ complex is impaired in DNA binding and acts as a dominant negative activator of flowering at higher temperatures. Our results demonstrate the importance of temperature-dependent alternative splicing in modulating the timing of the floral transition in response to environmental change. Overall design ChIP-seq A. thaliana FLM (3 replicates for gFLM and 2 replicates for FLM splice variants)
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