Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 525401
Title Quantitatieve permeabiliteitsbepalingen
Author(s) Wieringa, K.T.
Source University. Promotor(en): N.L. Soehngen. - Wageningen : Veenman - 81
Publication type Dissertation, internally prepared
Publication year 1928
Keyword(s) permeabiliteit - membranen - cytologie - saccharomyces cerevisiae - cum laude - permeability - membranes - cytology
Categories Cellular Biology / Membranes
Abstract Permeability of cell membranes of pressed baker's yeast was estimated by estimating the decline in concentration of the permeating substance. Therefore it was necessary to know the amount of water imbibed in the cell walls, the free water in the cells, and the imbibed water of the dispersed colloidal phase (protoplasm) in the cells.
With correction for the dispersed colloidal phase, the yeast cells behaved according to the law of Boyle Mariotte van 't Hoff. The uptake of substances by yeast cells was estimated chemically. Inorganic cations and anions were taken up according to lyotropic range, Ca 2+ < Mg 2+ < Na + < K + < NH 4+ and S0 42- < Cl - < N0 3- ,

Uptake of organic acids depended closely on their degree of dissociation. Of the sugars tested lactose did not permeate, whereas glucose was rapidly adsorbed by the cell surface. Some substances were adsorbed by the disperse phase (K + and urea).
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