Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 525947
Title Lytic polysaccharide monooxygenases from Myceliophthora thermophila C1
Author(s) Frommhagen, Matthias
Source University. Promotor(en): Harry Gruppen; Willem van Berkel, co-promotor(en): Mirjam Kabel. - Wageningen : Wageningen University - ISBN 9789463436434 - 192
Department(s) Food Chemistry Group
VLAG
Biochemistry
Publication type Dissertation, internally prepared
Publication year 2017
Abstract

Current developments aim at the effective enzymatic degradation of plant biomass polysaccharides into fermentable monosaccharides for biofuels and biochemicals. Recently discovered lytic polysaccharide monooxgygenases (LPMOs) boost the hydrolytic breakdown of lignocellulosic biomass, especially cellulose, due to their oxidative mechanism. At the beginning of this thesis, only few LPMOs were characterized and many aspects related to their catalytic performance were unknown. Hence, in this thesis, we investigated AA9 LPMOs from Myceliophthora thermophila C1. This fungus encodes 22 putative AA9 LPMOs and we hypothesized that these enzymes differ in their substrate preference and mode of action towards plant cell wall polysaccharides.

We demonstrated that MtLPMO9A oxidizes xylan associated to cellulose, which is the only published LPMO comprising this capability known so far. We also showed that MtLPMOs from M. thermophila C1 differ in their substrate preference and C1-/C4-regioselectivity. Moreover, we described the use of reversed phase (RP)-UHPLC in combination with non-reductive 2-aminobenzamide (2-AB) labeling to separate and identify C4-oxidized gluco-oligosaccharides.

All characterized MtLPMOs differ in their reducing agent preference. The highest amount of non-oxidized and oxidized gluco-oligosaccharides from cellulose were released by MtLPMOs in the presence of reducing agents with a 1,2-benzenediol or 1,2,3-benzenetriol moiety. The latter compounds can be formed from lignin-building blocks by using polyphenol oxidases (PPOs), such as MtPPO7 from M. thermophila C1, which boost the LPMO-driven lignocellulose oxidation. Sequence analysis of genomes of 336 Ascomycota and 208 Basidiomycota revealed a high correlation between MtPPO7-like and AA9 LPMO-like genes. Finally, a β-glucosidase-assisted method was developed to quantify the catalytic performance of the C1-oxidizing MtLPMO9B and MtLPMO9D. The catalytic performance of both MtLPMOs was strongly dependent on pH and temperature. Notably, pH mainly affected the reducing agent dependency whereas temperature influenced the operational stability of both MtLPMOs.

In summary, our study contributed to the further understanding of LPMO-driven lignocellulose degradation.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.