We report the chromosome and plasmid sequences of a spontaneous nalidixic acid resistant derivative of the ESBL-producing E. coli strain KV7. E. coli KV7 was originally isolated from a healthy pig that was treated prophylactically with ceftiofur. KV7 belongs to serotype O27 and phylogenetic group D, and has been used in experimental infection of piglets to study the effect of beta-lactam antibiotics on selection and persistence of ESBL-producing E. coli. A combination of PacBio and Illumina sequencing allowed us to provide a SNP-corrected single-contig assembly for the chromosome and each of six plasmids.
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