Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 530029
Title The viral susceptibility of individuals is affected by their genetic make-up
Author(s) Sluijs, Lisa van
Event Zoology 2017, Wageningen, 2017-11-24/2017-11-24
Department(s) Laboratory of Nematology
Publication type Unpublished lecture
Publication year 2017
Abstract Viral susceptibility differs between individuals due to genetic and environmental differences. Currently, the molecular mechanisms underlying differences in individual susceptibility are poorly understood. Here, we use the model organism Caenorhabditis elegans to unravel molecular pathways affected by genetic polymorphisms that lead to differences in susceptibility of an individual. Two distinct genotypes, N2 and CB4856, differ in viral susceptibility to infection by Orsay virus. Here, we used quantitative trait loci (QTL) mapping in N2xCB4856 recombinant inbred lines (RILs) in order to identify loci affecting susceptibility. We found two genomic loci correlated with viral susceptibility. Introgression lines (ILs) containing single genetic fragments of one parental strain in the genetic background of the other strain were used to finemap the genetic loci. After infection of a panel of ILs, the QTL region could be narrowed down to a region containing about 30 polymorphic genes. We selected genes using literature data and databases that predict the structure and function of proteins. The selected candidate polymorphisms found by the analysis will be targeted by CRISPR-Cas9 to exchange specific alleles between the parental strains. In the end, this approach can help to unravel how genetic polymorphisms can determine the viral susceptibility of an individual.
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