Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 531359
Title The need for standardisation : Exemplified by a description of the diversity, community structure and ecological indices of soil nematodes
Author(s) Griffiths, B.S.; Groot, G.A. de; Laros, I.; Stone, D.; Geisen, S.
Source Ecological Indicators 87 (2018). - ISSN 1470-160X - p. 43 - 46.
DOI http://dx.doi.org/10.1016/j.ecolind.2017.12.002
Department(s) Alterra - Animal ecology
Laboratory of Nematology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Biodiversity - DNA extraction - Metabarcoding - Microscopy - Molecular approaches - Nematodes - Standardisation
Abstract Molecular approaches are offering a supplement to, or even the possibility of replacing morphological identification of soil fauna, because of advantages for throughput, coverage and objectivity. We determined ecological indices of nematode community data from four sets of duplicate soil cores, based on morphological identification of nematodes after elutriation from 200 g soil and high throughput sequencing (HTS) targeting nematodes both after being elutriated from soils and DNA extracted directly from 10 g soil. HTS (at genus and species level) increased the taxonomic resolution compared to morphology (at family level). DNA extracted from elutriated nematodes identified more nematode taxa than when extracted from soil, due to an enrichment in nematode sequences. Each method also gave a different ecological footprint for the nematode community. Standardisation to previously determined indices based on morphological identification is needed in order to provide more meaningful information about soil quality and for ecological monitoring.
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