|Title||BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells|
|Author(s)||Hartog, C.G. den; Osch, Thijs L.J. van; Vos, Martijn; Meijer, B.; Savelkoul, H.F.J.; Neerven, R.J.J. van; Brugman, S.|
|Source||European journal of immunology 48 (2018)2. - ISSN 0014-2980 - p. 283 - 292.|
Cell Biology and Immunology
Human and Animal Physiology
|Publication type||Refereed Article in a scientific journal|
|Abstract||Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood B cells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naïve B cells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature B cells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.