Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 532633
Title Single-molecule protein sequencing through fingerprinting: computational assessment
Author(s) Yao, Y.; Docter, M.W.; Ginkel, H.G.T.M. van; Ridder, D. de; Joo, C.
DOI http://dx.doi.org/10.4121/uuid:08a078fc-9d4c-44d1-88c9-9cb412b83a46
Department(s) Bioinformatics
EPS
Publication type Dataset
Publication year 2018
Keyword(s) computational biophysics - proteomics - single-molecule biophysics
Abstract Proteins are vital in all biological systems as they constitute the main structural and functional components of cells. Recent advances in mass spectrometry have brought the promise of complete proteomics by helping draft the human proteome. Yet, this commonly used protein sequencing technique has fundamental limitations in sensitivity. Here we propose a method for single-molecule (SM) protein sequencing. A major challenge lies in the fact that proteins are composed of 20 different amino acids, which demands 20 molecular reporters. We computationally demonstrate that it suffices to measure only two types of amino acids to identify proteins and suggest an experimental scheme using SM fluorescence. When achieved, this highly sensitive approach will result in a paradigm shift in proteomics, with major impact in the biological and medical sciences.
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