Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 533677
Title Regulation of intercellular target of monopteros 7 protein transport in the arabidopsis root
Author(s) Lu, Kuan Ju; Rybel, Bert De; Mourik, Hilda Van; Weijers, Dolf
Source Development 145 (2018)2. - ISSN 0950-1991
DOI http://dx.doi.org/10.1242/dev.152892
Department(s) Biochemistry
EPS
Biometris (WU MAT)
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Cell-cell communication - Embryogenesis - Plasmodesmata - Protein transport - RAM - TMO7
Abstract Intercellular communication coordinates hypophysis establishment in the Arabidopsis embryo. Previously, TARGET OF MONOPTEROS 7 (TMO7) was reported to be transported to the hypophysis, the founder cell of the root cap, and RNA suppression experiments implicated its function in embryonic root development. However, the protein properties and mechanisms mediating TMO7 protein transport, and the role the movement plays in development remained unclear. Here, we report that in the post-embryonic root, TMO7 and its close relatives are transported into the root cap through plasmodesmata in a sequence-dependent manner. We also show that nuclear residence is crucial for TMO7 transport, and postulate that modification, potentially phosphorylation, labels TMO7 for transport. Additionally, three novel CRISPR/Cas9-induced tmo7 alleles confirmed a role in hypophysis division, but suggest complex redundancies with close relatives in root formation. Finally, we demonstrate that TMO7 transport is biologically meaningful, as local expression partially restores hypophysis division in a plasmodesmal protein transport mutant. Our study identifies motifs and amino acids that are pivotal for TMO7 protein transport, and establishes the importance of TMO7 in hypophysis and root development.
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