Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 533700
Title Colorful packages : fluorescent proteins in complex coacervate core micelles
Author(s) Nolles, Antsje
Source University. Promotor(en): Willem van Berkel, co-promotor(en): Jan Willem Borst; Mieke Kleijn. - Wageningen : Wageningen University - ISBN 9789463437226 - 175
Department(s) Physical Chemistry and Soft Matter
Publication type Dissertation, internally prepared
Publication year 2018
Availibility Full text available from 2019-02-14

This thesis explores the encapsulation of fluorescent proteins (FPs) into complex coacervate core micelles (C3Ms) and features the impact of this encapsulation on the biophysical properties of the FPs. In total eight different FPs were investigated originating from two different classes (Hydrozoa: SBFP2, mTurquoise2, EGFP, mEGFP, and SYFP2; and Anthozoa: mKO2, mCherry, and TagRFP), thereby covering the whole visible spectrum. As enveloping material the diblock copolymer poly(2-methyl-vinyl-pyridinium)n-b-poly(ethylene-oxide)m (P2MVPn-b-PEOm) of two different lengths (P2MVP41-b-PEO205 and P2MVP128-b-PEO477) was used. The research was focused on the formation, composition, dynamics, and stability of the FP-containing C3Ms, but it also gave us insights into the structural and spectral properties of the encapsulated FPs. We showed the successful encapsulation of about 500 EGFP molecules per C3M using both diblock copolymers. This high amount of FPs per C3M promoted dimerization of EGFP, resulting in a somewhat stronger acid character of its chromophore. By using seven other FPs, the effect of encapsulation on the structure and spectral properties of these proteins was systematically investigated. Hydrozoa FPs were more efficiently encapsulated than Anthozoa FPs, and the latter proteins were subject to di- or tetramerization in C3Ms. Finally, fast exchange dynamics of C3Ms were detected using FRET. Combining the insights presented in this thesis with sophisticated protein engineering and bioconjugation procedures, may lead in the near future to C3M-based protein nanoparticles that can be used for food and pharmaceutical applications.

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