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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 533857
Title Urea production by yeasts other than Saccharomyces in food fermentation
Author(s) Wu, Qun; Cui, Kaixiang; Lin, Jianchun; Zhu, Yang; Xu, Yan
Source FEMS Yeast Research 17 (2017)7. - ISSN 1567-1356
DOI https://doi.org/10.1093/femsyr/fox072
Department(s) Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract Urea is an important intermediate in the synthesis of carcinogenic ethyl carbamate in various food fermentations. Identifying urea-producing microorganisms can help control or reduce ethyl carbamate production. Using Chinese liquor fermentation as a model system, we identified the yeasts responsible for urea production. Urea production was positively correlated to the yeast population (R = 0.523, P = 0.045), and using high-throughput sequencing, we identified 26 yeast species. Partial least squares regression and correlation analysis indicated that Wickerhamomyces anomalus was the most
important yeast to produce urea (variable importance plot = 1.927; R = 0.719, P = 0.002). Besides, we found that in W. anomalus the CAR1 gene (responsible for urea production) was 67% identical to that of Saccharomyces cerevisiae.
Wickerhamomyces anomalus produced more urea (910.0 μg L−1) than S. cerevisiae (300.1 μg L−1). Moreover, urea production
increased to 1261.2 μg L−1 when the two yeasts were co-cultured in a simulated fermentation, where the transcription
activity of the CAR1 gene increased by 140% in W. anomalus and decreased by 40% in S. cerevisiae. Our findings confirm
that a yeast other than Saccharomyces, namely W. anomalus, contributes more to urea formation in a simulated s
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