Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 533999
Title Aged gut microbiota contributes to systemical inflammaging after transfer to germ-free mice
Author(s) Fransen, Floris; Beek, A.A. van; Borghuis, Theo; Aidy, Sahar El; Hugenholtz, F.; Gaast-de Jongh, Christa van der; Savelkoul, H.F.J.; Jonge, Marien I. De; Boekschoten, M.V.; Smidt, H.; Faas, Marijke M.; Vos, Paul de
Department(s) Cell Biology and Immunology
Microbiological Laboratory
WIAS
VLAG
Chair Nutrition Metabolism and Genomics
WIMEK
Publication type Dataset
Publication year 2018
Keyword(s) Mus musculus - GSE104063 - PRJNA408136
Abstract Advanced age is associated with chronic low-grade inflammation, which is usually referred to as inflammaging. Elderly are also known to have an altered gut microbiota composition. However, whether inflammaging is a cause or consequence of an altered gut microbiota composition is not clear. In this study gut microbiota from young or old conventional mice was transferred to young germ-free mice. Four weeks after gut microbiota transfer immune cell populations in spleen, Peyer’s patches, and mesenteric lymph nodes from conventionalized germ-free mice were analyzed by flow cytometry. In addition, whole-genome gene expression in the ileum was analyzed by microarray. Gut microbiota composition of donor and recipient mice was analyzed with 16S rDNA sequencing. Here we show by transferring aged microbiota to young germ-free mice that certain bacterial species within the aged microbiota promote inflammaging. This effect was associated with lower levels of Akkermansia and higher levels of TM7 bacteria and Proteobacteria in the aged microbiota after transfer. The aged microbiota promoted inflammation in the small intestine in the germ-free mice and enhanced leakage of inflammatory bacterial components into the circulation was observed. Moreover, the aged microbiota promoted increased T cell activation in the systemic compartment. In conclusion, these data indicate that the gut microbiota from old mice contributes to inflammaging after transfer to young germ-free mice.
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