Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 534128
Title Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens
Author(s) Arts, J.A.J.; Bovenhuis, H.; Lammers, A.; Poel, J.J. van der; Parmentier, H.K.
Source Vaccine 36 (2018)11. - ISSN 0264-410X - p. 1444 - 1452.
DOI http://dx.doi.org/10.1016/j.vaccine.2018.01.063
Department(s) Adaptation Physiology
WIAS
Animal Breeding and Genetics
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Breeding - Chicken - General disease resistance - Keyhole limpet hemocyanin (KLH) - Natural antibody - Specific antibody
Abstract NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.
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