Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 534178
Title Label-free biosensor detection of endocrine disrupting compounds using engineered estrogen receptors
Author(s) Spina, Rita La; Ferrero, Valentina E.V.; Aiello, Venera; Pedotti, Mattia; Varani, Luca; Lettieri, Teresa; Calzolai, Luigi; Haasnoot, Willem; Colpo, Pascal
Source Biosensors 8 (2017)1. - ISSN 2079-6374
DOI https://doi.org/10.3390/bios8010001
Department(s) VLAG
RIKILT - BU Authenticity & Nutrients
Publication type Refereed Article in a scientific journal
Publication year 2017
Keyword(s) 17β-estradiol - Amplification - Estrogen receptor - Label-free assay - Surface plasmon resonance - Water
Abstract Endocrine Disrupting Compounds (EDCs) are chemical substances shown to interfere with endogenous hormones affecting the endocrine, immune and nervous systems of mammals. EDCs are the causative agents of diseases including reproductive disorders and cancers. This highlights the urgency to develop fast and sensitive methods to detect EDCs, which are detrimental even at very low concentrations. In this work, we propose a label-free surface plasmon resonance (SPR) biosensor method to detect specific EDCs (17 β-estradiol (E2), ethinyl-estradiol, 4-nonylphenol, tamoxifen) through their binding to estrogen receptor alpha (ERα). We show that the use of rationally designed ERα (as bio-recognition element) in combination with conformation-sensitive peptides (as amplification agent, resulting in increased responses) enables the detection of low parts per billion (ppb) levels of E2. As a proof of concept, this bioassay was used to detect E2 in (spiked) real water samples from fish farms, rivers and the sea at low ppb levels after concentration by solid phase extraction. In addition, the present SPR assay that combines a conformation-sensitive peptide with an array of ERα mutants is very promising for the assessment of the risk of potential estrogenic activity for chemical substances.
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