Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 534465
Title The rumen microbiome : An underexplored resource for novel antimicrobial discovery
Author(s) Oyama, Linda B.; Girdwood, Susan E.; Cookson, Alan R.; Fernandez-Fuentes, Narcis; Privé, Florence; Vallin, Hannah E.; Wilkinson, Toby J.; Golyshin, Peter N.; Golyshina, Olga V.; Mikut, Ralf; Hilpert, Kai; Richards, Jennifer; Wootton, Mandy; Edwards, Joan E.; Maresca, Marc; Perrier, Josette; Lundy, Fionnuala T.; Luo, Yu; Zhou, Mei; Hess, Matthias; Mantovani, Hilario C.; Creevey, Christopher J.; Huws, Sharon A.
Source npj Biofilms and Microbiomes 3 (2017)1. - ISSN 2055-5008
DOI http://dx.doi.org/10.1038/s41522-017-0042-1
Department(s) Microbiological Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2017
Abstract Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.
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