Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 535811
Title Type 2 diabetes-related proteins derived from an in vitro model of inflamed fat tissue
Author(s) Klooster, Jean Paul ten; Sotiriou, Alexandros; Boeren, Sjef; Vaessen, Stefan; Vervoort, Jacques; Pieters, Raymond
Source Archives of Biochemistry and Biophysics 644 (2018). - ISSN 0003-9861 - p. 81 - 92.
DOI http://dx.doi.org/10.1016/j.abb.2018.03.003
Department(s) Biochemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Adipocyte - AdipoQ - AUH - CSNK2A2 - Haptoglobin - IL6 - LPS - Macrophage - NAGK - NNMT - pCYT2 - STK39 - TNFα
Abstract Currently, there is a worldwide increase of patients with type 2 diabetes (T2D). During the progression of healthy obese to T2D status, there is an influx of immune cells, in particular macrophages, into visceral adipose tissue, accompanied by an increase of inflammatory cytokines, such as, IL6, TNFα and Hp. To get a better insight in the underlying mechanisms, we performed a quantitative LCMS analysis on a modified in vitro assay, combining 3T3L1 adipocytes and activated RAW264.7 macrophages, thus mimicking inflamed adipose tissue. Clinically known proteins, e.g. IL6, TNFα AdipoQ, complement factor C3, B and D were identified, thus confirming the assay. In addition, we found 54 new proteins that can potentially be used for research into the mechanism of T2D. Comparison of our results to a study on human visceral fat of obese non-diabetic and obese diabetic subjects, indicated that AUH, NAGK, pCYT2, NNMT, STK39 and CSNK2A2 might indeed be linked to insulin resistance in humans. Moreover, the expression of some of these genes was also altered in human blood samples at early or later stages of insulin desensitization. Overall, we conclude that the direct contact co-culture of 3T3L1 adipocytes with activated macrophages could be a mechanistically relevant and partially translational model of inflamed visceral adipose tissue.
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