Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 535825
Title RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana
Author(s) Fristedt, Rikard; Hu, Chen; Wheatley, Nicole; Roy, Laura M.; Wachter, Rebekka M.; Savage, Linda; Harbinson, Jeremy; Kramer, David M.; Merchant, Sabeeha S.; Yeates, Todd; Croce, Roberta
Source The Plant Journal 94 (2018)1. - ISSN 0960-7412 - p. 146 - 156.
DOI https://doi.org/10.1111/tpj.13849
Department(s) Horticulture and Product Physiology Group
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Abscisic acid - Atg5 g51110 - Chloroplast - RAF2 - RuBisCO - RuBisCO aggregation - RuBisCO assembly factor - SDIRIP1
Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose-1,5-bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to pterin-4α-carbinolamine dehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD-like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana.
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