Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 535826
Title Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (Crangon crangon)
Author(s) Eynde, Benigna Van; Christiaens, Olivier; Delbare, Daan; Cooreman, Kris; Bateman, Kelly S.; Stentiford, Grant D.; Dullemans, Annette M.; Oers, Monique M. van; Smagghe, Guy
Source Journal of Invertebrate Pathology 153 (2018). - ISSN 0022-2011 - p. 195 - 202.
DOI https://doi.org/10.1016/j.jip.2018.03.006
Department(s) PPO/PRI Biointeractions and Plant Health
Laboratory of Virology
PE&RC
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Aquaculture - CcBV - Crustacean - Diagnostic - Disease - Nudiviridae
Abstract Crangon crangon bacilliform virus (CcBV) was first discovered in 2004 in European brown shrimp (Crangon crangon) caught along the English coast. This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of C. crangon as an internal amplification control. The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for C. crangon, respectively. Sequencing of the resulting amplicons confirmed the specificity of this PCR assay and sequence analysis of the lef-8 fragment revealed amino acid identity percentages ranging between 31 and 42% with members of the Nudiviridae, proposing that CcBV may reside within this family.Finally, the duplex PCR assay was applied to samples of C. crangon hepatopancreas tissue collected along the Belgian coast to screen for the presence of CcBV. The prevalence of CcBV averaged 87%, which is comparable to previous reports of high prevalence, based upon histological analysis, in shrimp collected along the English coast. Development of a specific and sensitive PCR assay to detect CcBV will provide a useful tool for future aquaculture and research programs involving C. crangon.
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